Inducing RNAi in <i>Radopholus similis</i> by soaking with <i>Rs-crt</i> dsRNA.

<p>(A) Expression of <i>Rs-crt</i> mRNA in <i>R</i>. <i>similis</i> treated with <i>Rs-crt</i> dsRNA. Bars indicate the standard errors of the mean (n = 3). (B) Number of nematodes on carrot disks 50 d after the inoculation of 30 females. Bars indicate the standard errors of the mean data (n = 5). CK: untreated nematodes; G12-G48: nematodes treated with <i>egfp</i> dsRNA for 12 h, 24 h, 36 h and 48 h, respectively; R12-R48: nematodes treated with <i>Rs-crt</i> dsRNA for 12 h, 24 h, 36 h and 48 h, respectively. Different letters indicate significant differences (<i>p</i><0.05) between treatments.