LASP1 knockdown affects cell migration and proliferation.

<p>For analysis of proliferation, melanoma cell lines transfected with LASP1 siRNA and control siRNA were seeded in 48-well-plates and incubated for 72h. Cells were counted with MTT viability assay and by Neubauer chamber. Bars, SEM; * Student´s <i>t</i> test, p<0.05; <i>versus</i> control. For investigation of adhesion properties, LASP1-depleted cells were seeded in 48-well-plates and incubated for 4h. Non-adherent cells were washed-off, adherent cells were counted with Promega Glo. Bars, SEM; n.s. p≥0.05, versus control. For migration analysis, LASP1-depleted cells were seeded in modified Boyden chambers and incubated for 6h. Migrating cells were fixed with paraformaldehyde and stained with crystal violet. The absorbance was measured. Bars, SEM; *** Student´s <i>t</i> test, p≤0.001; <i>versus</i> control. A representative Western blot for LASP1 knockdown (~75%) in both cell lines is shown.