Antibody reactivity against viruses expressing parental or non-parental HAs using serum samples from pigs immunized with the TX98-129 IIV.

<p>Sera were collected at 14 days after secondary inoculation of pigs with the candidate TX98-129 IIV vaccine. (A) Serum antibody HAI titers are defined as the reciprocal of the final serum dilution where inhibition of hemagglutination was observed. (B) Serum antibody MN titers are defined as the reciprocal of the final serum dilution where OD₄₉₀ was below 50% of positive control wells, using 100 TCID₅₀ virus inoculum (confirmed by back-titration). In both panels, serum samples with a titer below the detectable limit of the assay (initial serum dilution of 1:10) were assigned a value of 5 for graphical representation and statistical analyses. Viruses expressing non-parental HA proteins are abbreviated (A/North Carolina/18161/2002: NC02; A/swine/Iowa/1/1985: IA85; A/swine/Iowa/40766/1992: IA92; A/swine/Germany/2/1981: GE81; A/New Caledonia/20/99: NC99) and shown with clade representation. Significance between vaccinated vs. unvaccinated for all viruses was determined using a Mann Whitney nonparametric test (*p<0.05).