Nef-induced IFNβ production concurs to Nef-dependent iNOS induction.

<p>(<b>A</b>) BV-2 cells were treated for the indicated time with myr⁺Nef_SF2 (100 ng/ml) in the presence of anti-IFNβ neutralizing antibodies as described [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130189#pone.0130189.ref036" target="_blank">36</a>]. iNOS mRNA was analyzed as reported in materials and methods section. Dark gray bars: 4 h treatment; light gray bars: 8 h treatment. (<b>B</b>) Cells were treated as in (<b>A</b>) for the indicated time and iNOS expression evaluated by Western Blot. β-Tubulin expression was used as an internal loading control. (<b>C</b>) Cells were treated as in (<b>A</b>) for 24 h, supernatants were then collected and NO₂^- content measured using the Griess colorimetric assay.