Relative Oct4 and Thy1 transcripts in early embryos.

<p>A and B, relative transcripts of Oct4 and Thy1 at 1-cell, 2-cell, 4-cell, 8-cell and blastocyst stages of IVF, NT-CON, NT-AZA and NT-TSA embryos, respectively. 5-aza-dC or TSA appropriately promoted the expression of Oct4 and silenced the transcription of Thy1 in cloned embryos. The transcript abundance in MII oocytes (A) or cloned embryos at 2 h post activation (B) was considered to be the control. The data were expressed as mean ± SEM. ^a-cValues at a given stage for the same gene with different superscripts differ significantly (P<0.05).