Thy1 methylation statuses in early embryos.

<p>A, the methylation statuses of Thy1 at 1-cell, 2-cell, 4-cell, 8-cell and blastocyst stages of IVF, NT-CON, NT-AZA and NT-TSA embryos, B, the methylation levels of Thy1 in the IVF, NT-CON, NT-AZA and NT-TSA groups, and C, the methylation levels of Thy1 at different stages of IVF, NT-CON, NT-AZA and NT-TSA embryos. Cloned embryos displayed the disrupted methylation pattern of Thy1, while 5-aza-dC or TSA promoted Thy1 remethylation in cloned embryos. Black or white circles indicate methylated or unmethylated CpG sites, respectively, and gray circles represent mutated and/or SNP variation at certain CpG sites. ^a-cValues in the same group or at a given stage with different superscripts differ significantly (P<0.05).