Expression and purification of recombinant proteins.
- Dongliang Fei (753842)
- Haochun Zhang (753843)
- Qingyun Diao (753844)
- Lili Jiang (65114)
- Qiang Wang (32383)
- Yi Zhong (153468)
- Zhaobin Fan (753845)
- Mingxiao Ma (753846)
Publication date
June 2015
DOIAbstract
<p>A, B, and C represent roVP1, roVP2, and roVP3 proteins, respectively. SDS-PAGE analysis of roVP1, roVP2, and roVP3 proteins were as follows: Lane 1, low molecular weight protein marker; Lane 2, pGEX-6P-1 vector after induction; Lane 3, wild type gene expression after induction; Lane 4, recoding gene expression after induction; Lane 5, purification of expressed proteins using GST-agarose affinity columns.</p
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