The expression of PCNA in tumor tissue.

<p>(A) Immunohistochemical staining analysis. PCNA-positive cells in the sections are stained brown. The nuclei are stained blue. Representative figures from each group are shown; n = 6; Magnification, × 400; Scale bars = 50 μm. (B) The corresponding quantitative analysis results of PCNA are shown in (A). Five fields from each tumor tissue section were randomly selected for the calculation of the IOD value of the positive region through IPP software, which indicated the amount of antigen expression. (C) Western blot analysis. Total protein from the tumor tissues was extracted. PCNA was detected with β-actin as an internal control. n = 3. (D) The quantitative analysis results of PCNA are shown in (D). The intensity of each strip was analyzed by ImageJ software. The average intensities of PCNA were standardized to β-actin. Error bars, mean ± SD; ns, not significant; *** <i>p</i> <0.001.