OR expression in the human TG and DRG.

<p><b>A</b> The bar diagram shows the summarized expression (sFPKM) of detected OR transcripts. The sFPKM values of OR gene transcripts in the TG and DRG are in the same range as the summarized expression values found in testis tissue. <b>B</b> The heatmap shows FPKM values for the 30 most abundant OR transcripts found in the TG 1 sample compared with other TG and DRG samples and reference tissues (brain, colon, liver, lung, s.muscle, and testis). Darker colors indicate higher FPKM values, and white indicates the absence of detectable transcripts. The ORs were sorted according to the FPKM values found in the TG 1 sample. As described in [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0128951#pone.0128951.ref041" target="_blank">41</a>], the FPKM values of OR2A4 and OR2A7 as well as that of OR2A1 and OR2A42, which share high sequence similarity (99–100%), were analyzed together. <b>C</b> Validation of RNA-Seq results using semi qRT-PCR. The diagram shows the summarized semi qRT-PCR compared with 3 ng of genomic DNA. We investigated 22 different ORs that showed either high, moderate, or low expression in TG 1. <b>D</b> Microarray analysis of the ORs in TG 1. Intensity values for the TG 1 sample were normalized to the reference tissues (liver, heart, s. muscle, skin). Given are all ORs with ratios >1.5. ORs were also detected by RNA-Seq as indicated by red frames. t-test: *p<0.05; **p<0.00013 (after Bonferroni correction).