Phenotypes of <i>in vitro</i> cultured cell types at D18.

<p>(A) Schematic view of whole EET surrounding the embryonic disc (ED). Chorion (ch) is composed of ectoderm (or Trophoblast; magenta line) and ExMesoderm (red), yolk sac (ys) of ExMesoderm and ExEndoderm (green). (B) bTCs, bXMCs, and bXECs were primarily cultured on collagen (colIV) or plastic (where plastic means a tissue-culture-treated surface). Co-immunofluorescence is shown at 16h, 72h, or a week of culture with the antibodies used <i>in vivo</i> (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0127330#pone.0127330.g001" target="_blank">Fig 1</a>: Trophoblast—FURIN, ExMesoderm—VIM, or ExEndoderm—AFP) as well as phase-contrast images of each cell type after 72h of culture. (C) Immunodetection of cytoskeleton organization and cell proliferation with pan-Keratins (KRTs) and Ki67 respectively, after 1 week of culture. Scale bar: 10 μm.