Suppression of PC expression in MDA-MB-231 retarded proliferation both in glutamine-nourished and glutamine-depleted conditions.

<p>MDA-MB-231 cells were transiently transfected with PC or scrambled control siRNAs. At 48 h post transfection cells were trypsinized, re-plated and grown in the presence of 0 mM or 4 mM glutamine for 7 days. The proliferation rate of the PC knocked down (PC siRNA) and the control MDA-MB-231 cell lines (Control) grown in the medium containing 4 mM (A) or 0 mM (C) glutamine. The relative expression of PC mRNA in the knocked down MDA-MB-231 cells grown in the presence of 4 mM (B) or 0 mM (D) glutamine throughout the assay. The results are means obtained from two independent experiments, each in triplicate. The statistical analysis was conducted using ANOVA test where *<i>P</i> < 0.05, **<i>P</i> < 0.01, ***<i>P</i> < 0.001.