Southern and Northern blot analysis of HBV replication and transcription in vitro and in vivo.

<p>(a) Detection of HBV DNA replicative intermediates 7 days post pSSV9-1.2HBV transfected to Huh7.5.1 cell. (b) Intermediates of HBV DNA replication 1, 3, 6 months after AAV8-1.2HBV injection. The relaxed-circular(RC), double-stranded linear (DS) and single-stranded (SS)DNAs were indicated.(c) Northern blotting to detect 3.5kb pregenomic, 2.4kb and 2.1kb mRNAs in the livers post injection. The amount of RNA bound to the membrane was normalized by housekeeping gene (GAPDH). HBV(-) mice, PBS injected mice.