Sequence alignments of wild-type rs2275558, c.934C>T in AMH and rs34072914 of eight species.

<p><b>(A)</b> Nucleic acid sequence alignments of rs227558 in PBX1, c.934C>T in AMH and rs34072914 in WNT9B of eight different species. (<b>B)</b> Amino acid sequence alignment of PBX1 p.21G>S and AMH p.312R>C of eight different species. ClustalW program was used to perform alignments. The bold letters indicate the 3 variants of interest. The hyphen indicates that the nucleotide base or amino acid is consistent with that in the human wild-type gene or protein, respectively. Zero means there is no corresponding sequences. Rs2275558 in PBX1 was consistently present in all 8 vertebrates, and rs34072914 in WNT9B and c.934C>T in AMH presented relatively conservative in mammals.