Proliferation of granulosa cells in FST288-treated ovaries.

<p>Ovaries at 17.5 dpc were cultured alone (as a control) or with 500 ng/mL FST288 for BrdU incorporation assays. After 4 days of culture, when compared to granulosa cells of control ovaries (A-C, arrows; BrdU-positive granulosa cells), BrdU incorporation into granulosa cells of FST288-treated ovaries was reduced (D-F, arrows; BrdU-positive granulosa cells), particularly near the ovarian cortex. Similarly, BrdU incorporation into granulosa cells near the periphery of FST288-treated ovaries (J-L) was reduced relative to granulosa cells of control ovaries after 7 days of culture (G-I, arrows; BrdU-positive granulosa cells). Almost all of the somatic cells near the ovarian cortex were BrdU-negative (J-L). Scale bars: 40 μm (A-L).