NaCl treatment increased Aβ levels in culture medium.

<p>HEK293 stable cells overexpressing APP (A and B) or C99 (C and D), or primary neurons from APP/PS1 mouse (E and F) were incubated with normal or NaCl medium for 24h and the medium were replaced by normal medium for another 12h. Then Aβ levels in the medium were measured by ELISA (n = 3). Data were presented as means ± s.e.m. of indicated numbers of independent experiments. **P<0.01, ***P<0.001.