Expression pattern of tdTomato in the mouse retina.

<p><b>A:</b> Top view of a retinal wholemount preparation showing the distribution of tdTomato-expressing cells within the retina of a 2.5 month old animal. Scale bar: 100 μm. <b>B-D:</b> Transverse section of the retina stained with DAPI to stain cellular nuclei to visualize retinal layering (B). TdTomato-expressing cells (C) were found in inner nuclear layer (INL) and ganglion cell layer (GCL). <b>D:</b> overlay of DAPI- (blue) and tdTomato- (red) fluorescence. Scale bar in D corresponds to 40 μm and applies to B-D. IPL: inner plexiform layer; OPL: outer plexiform layer; ONL: outer nuclear layer; RPE: retinal pigment epithelium. Red fluorescence within the RPE is due to autofluorescence.