Identification of GABAergic and glycinergic neurons in TgN(GAD65-tdTomato) x TgN(GlyT2-EGFP) double transgenic mice by several microscopic techniques.

<p><b>A:</b> Overview of a frontal brain slice of a double transgenic 30 day old mouse showing tdTomato (red) expressing GABAergic and EGFP (green) expressing glycinergic neurons. The image was acquired using epifluorescence. Scale bar: 1 mm. <b>B:</b> High magnification epifluorescence image. <b>C:</b> GABAergic and glycinergic cells expressing tdTomato or EGFP, respectively, can also be visualized using confocal imaging. <b>D:</b> Also by using 2-photon laser scanning microscopy, tdTomato- and EGFP-fluorescence can be observed allowing for unequivocal identification of GABAergic and glycinergic neurons, respectively. Scale bar in D corresponds to 40 μm and applies to B-D.