Transcription and translation of Xlr5c in mouse tissues and developing testes.

<p>(A) Position of primers used in RT-PCR experiments. Xlr5c mRNA is expressed exclusively in mouse testes and ovaries. (B) The Xlr5c transcript was first detected at 1 day post partum in postnatal mouse testes using RT-PCR. Expression levels increased gradually thereafter. (C) Two proteins of ~20 kDa and ~26 kDa were detected in testes using western blotting. The larger proteins correspond with Xlr5a and/or Xlr5b, and the smaller proteins correspond with Xlr5c. Gapdh mRNA and protein was used as an internal control.