Tumor clearance and effect of irradiation on tumor cell immunogenicity.

<p>(<b>A</b>, <b>B</b>) Representative mouse from untreated and combination therapy group showing growing and shrinking tumor respectively. All bio-luminescent images were analyzed under the same scale.(<b>C</b>, <b>D</b>) Irradiated Id2 knock down Neuro2a (IR-Id2kdN2a, n = 5) and wild type Neuro2a (IR-wtN2a, n = 5) were compared as whole tumor cell vaccine antigen source in combination with α-CTLA-4 antibody against AgN2a (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0129237#pone.0129237.g003" target="_blank">Fig 3A</a>). IR-Id2kdN2a vaccine was able to eradicate 60% of tumors in comparison to IR-wtN2a cells which had no effect on growth of the AgN2a tumors. All mice in (C) were sacrificed on day 20 due to large tumor burden. The parenthesis indicates number of mice that survived tumor free.