Immobilization of Enzymes via Microcontact Printing and Thiol–Ene Click Chemistry

This Communication describes a bioconjugation method for the generation of enzyme microarrays on surfaces using photochemical thiol–ene chemistry in combination with microcontact printing. Glucose oxidase and lactase were readily immobilized (i.e., printing time 2 min) on alkene terminated self-assembled monolayers on glass as demonstrated by X-ray photoelectron spectroscopy and fluorescence microscopy. Furthermore, the activity of both immobilized enzymes was confirmed in single enzyme as well as cascade transformations