Estimated <i>Puccinia striiformis</i> f. sp. <i>tritici</i> of 5 mg urediniospores at three storage conditions using the qPCR of DNA (dashed lines) and RNA (solid lines).

<p>Dashed lines with triangles, squares and circles represent DNA from dead urediniospores at ambient temperature, fresh urediniospores at ambient temperature and at -20°C, respectively. Solid lines with triangles, squares and circles represent RNA from dead urediniospores at ambient temperature, and fresh urediniospores at ambient temperature and at -20°C, respectively. The experiment was done three times. The vertical bar of each treatment at each time point represents the standard deviation of the three mean values from three experiments; significant treatment differences were based on the pooled residual error in the repeated measurement ANOVA. Treatments with different letters for the 12-days means were significantly different at <i>P</i> = 0.05. There were no significant differences among the treatments on 0, 4 and 8 days at <i>P</i> = 0.05.