Cytotoxic effects of 1,3,4-thiadiazolium derivatives on hepatocytes.

<p>A. MTT assay (the experimental conditions are described in the Materials and Methods section 2.5.1) The cells were seeded with or without 1,3,4-thiadiazolium derivatives at 25 for 18–24 h. The results were expressed as % of viability in comparison to control. B. LDH release assay (the experimental conditions are described in the Materials and Methods section 2.5.2). Under the same treatment conditions described above, LDH activity was measured in the supernatants. Data represent means of four different experiments in quadruplicate. The results were expressed as % of viability in comparison to control. ** and *** denotes values significantly different from the control or between the different treatments at <i>P</i>< 0.01 and <i>P</i>< 0.0001, respectively.