MSH2 knockout mutants are more resistant to oxidative stress generated by H₂O₂.

<p>(<b>A</b>) <i>T</i>. <i>brucei</i> wild type (WT), <i>msh2</i>+/-, <i>msh2</i>-/-, <i>mlh1</i>+/- and <i>mlh1</i>-/- procyclic form cells were grown in culture medium with 0 μM, 10 μM or 20 μM H₂O₂. Cell density was measured after 48 hours and plotted as the percentage survival of the H₂O₂ treated cells relative to untreated. (<b>B</b>) <i>T</i>. <i>cruzi</i> epimastigote WT, <i>msh2</i>+/- and <i>msh2</i>-/- cells were incubated with or without 75 μM H₂O₂ for 20 minutes in PBS 1x and then allowed to grow in LIT medium for 48 hours, after which cell viability was determined and plotted as percentage survival of the treated cells relative to untreated. Vertical lines show standard deviation. ***p<0.001, **p<0.01, *p<0.05: determined by one-way ANOVA with Bonferroni post-test of mutants relative to wild type cells; ns indicates no signifcant difference.