Deletion of <i>Kdm5b</i> in DCs leads to increased cytokine production and decreased Th2 pathology <i>in vivo</i>.

<p>(A) DCs were cultured from bone marrow of <i>Kdm5b</i>^f/f-CD11c-Cre⁺ mice and Cre^- controls and were infected with RSV. RNA was extracted after 24 hours and cytokine production measured by qPCR. (B) <i>Kdm5b</i>^f/f-CD11c-Cre⁺ mice and Cre^- controls were infected intranasally with RSV and the lungs were removed at 2 and 4 dpi. Lung tissue was homogenized and RNA extracted. Cytokine levels were measured by qPCR, and are expressed as fold change over infected Cre^- controls. (C) BMDCs were infected with RSV for 24 hours, and a ChIP assay was performed to determine the H3K4me3 status at the promoter regions of the <i>Ifnb</i>, <i>Tnf</i> and <i>Il6</i> genes. (D) Lungs were removed from mice at 2 and 4 dpi and viral mRNA levels for the F, G and N genes were measured by qPCR. (E) Infectious virus was determined by plaque assay at 2 and 4 dpi. (F) MLN were removed from mice at 8 dpi and were restimulated <i>in vitro</i> with RSV for 48 hours. Proteins in the supernatant were measured by bioplex assay. (G) Mice were infected with RSV for eight days, then lungs were removed and homogenized, and RNA was extracted. Transcripts of <i>Muc5ac</i> and <i>Gob5</i> were determined by qPCR. (H) Lung sections were stained with PAS to visualize mucus production at 8 dpi. Arrows indicate mucus production. (I) Mucus production was quantified from in lung sections on a scale of 1–4. *p<0.05, **p<0.01.