Scheme of IC for detection of anti-GLA antibodies in serum.

<p>A. Scheme of an IC chip which was designated for detecting anti-GLA antibodies in serum. Sample is dropped into the middle hole (arrow) and the reaction buffer including AP-labelled anti-human IgG in the reservoir unit is running on the membrane after a break by finger. C means control line and T does test line. B (1). The principle of IC for Aga-A and Aga-B. Mixture solution of serum and buffer including anti-GLA antibodies from serum of Fabry patients who received ERT with Aga-A and/or Aga-B was applied into the sample hole, and then the solution is running in the nitrocellulose membrane. B (2). After breaking the reservoir unit (Reservoir), the reaction buffer including AP-labelled anti-human IgG and substrate for AP which was stored in separate space in the reservoir unit is running on the membrane. B (3). If the sample has anti-GLA antibodies, the detection antibody on the membrane of IC captures the antibody/AP-labelled anti-human antibody/AP-substrate complex, and blue line appears on the test line (T). On the control line. The AP-labelled anti-human IgG antibody is trapped and makes blue color line, showing that the system works well (C). B. The same samples were applied to IC for Aga-A (upper lane) and Aga-B (lower lane), and the color line was scored from 0 to 8 after 15 min later. The color scale is shown in C.