Comparison of anti-GLA antibody levels in clinical samples using ELISA and IC.

<p>Sera from 29 Fabry patients (#1–29) and controls (Cont) were assayed by ELISA (left side) and IC (right side) as refer to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0128351#pone.0128351.t001" target="_blank">Table 1</a>. Control exhibits the average value of the data of healthy people (n = 20) used blank ELISA without both Aga-A neither Aga-B. According to the protocol, ELISA was performed using magnetic beads attached with Aga-A or Aga-B as antigens and the anti-GLA antibodies in each 100-fold diluted serum were measured with colorimetric methods of OD 450 nm using AP-labelled-anti-human IgG antibody and the substrate. The same serum were 5-fold diluted and applied to IC for Aga-A (black column) and Aga-B (white column), and judged by the color scale. The all data were shown as mean ± SD.