MTX altered ROS levels in platelets and its inhibition by NAC/NACA/Mito-TEMPO.

<p><b>A,</b> FACS analysis of ROS generation in washed platelets treated with MTX in presence or absence of NAC/NACA. <b>B,</b> GSH/GSSG ratio and (<b>C</b>) γ‑Glutamyltransferase activity. <b>D,</b> Effect of Mito-TEMPO on MTX induced ROS generation, expressed as percentage increase in DCF fluorescence. Effect of MTX in presence or absence of NAC/NACA on components of mitochondrial electron transport chain, (<b>E</b>) Complex I-NADH: ubiquinone oxidoreductase activity (<b>F</b>) Complex II-succinate: ubiquinone oxidoreductase activity (<b>G</b>) Complex III-coenzyme Q: cytochrome c-oxidoreductase activity and (<b>H</b>) Complex IV-cytochrome c oxidase activity. Values are presented as mean ± SEM (n = 5). p*/#< 0.05, p**/##< 0.01, p***/###< 0.001; *: significant compared to control. #: significant compared to MTX.