Clustering of subjects by abundances of <i>Haemophilus spp</i>., <i>Streptococcus spp</i>., and <i>Moraxella spp</i>.

<p>Subjects were grouped by hierarchical clustering on the basis of species-level percent 16S rRNA sequence abundances. Percent abundances are proportional to gray scaling. The upper heatmap presents data for baseline nasopharyngeal microbiomes and the lower heatmap presents data 180 days after vaccination. Subjects were classified into three basic groups on the basis of this clustering: A. <i>Streptococcus</i> dominant; B) <i>Moraxella</i> dominant; and C) Mixed <i>Streptococcus/Haemophilus</i> dominant. Solid lines connecting the two heatmaps indicate individuals that changed from cluster A to cluster B. Dotted lines indicate subjects that moved from cluster B to either cluster A or C. Black and gray boxes adjacent to dendogram designate vaccination group (PHiD-CV: 10-valent pneumococcal non-typeable <i>H</i>. <i>influenzae</i> protein-D conjugate vaccine; HAV: Hepatitis A vaccine).