Phenotyping of muscular dystrophy with <i>Gt(dmd-citrine)</i>^<i>ct90a</i>.

<p>(a,b). Dmd-citrine expression in control (a) and <i>dmd</i> morphants (b). Embryos in (a) and (b) were imaged with the same laser power and gain settings. (c) Western-blot of protein extracts from WT (lanes 1, 2) and <i>Gt(dmd-citrine)</i>^<i>ct90a</i> (lanes 3, 4) embryos with antibody to DMD and tubulin (loading control). Embryos for protein extracts were injected with control morpholino (lanes 1 and 3) and <i>dmd</i> morpholino (lanes 2 and 4, n = 3–6). Arrows point to size difference between Dmd and Dmd-citrine. (d,e) Widefield fluorescent image of 32hpf <i>ct90aGT</i> embryos untreated (d) and treated with heat-shocked at 6-somites stage. Arrow point to defects in Dmd-citrine expression visible between somites 16–25. (f-i) Heat-shocked embryos stained for Dmd (f,g; green) and anti-MHC (h,i; red) at 32hpf. Scale bars (d,e) 50μm (f-i) 20 μm.