Short-term hypoglycemic coma model.

<p>(A) Protocol for hypoglycemic loading and induction of the hyperglycemic state. Rapid-acting insulin was intraperitoneally administered at a dose of 15 IU/kg. After 2 or 10 min of the presence of an isoelectric electroencephalogram (EEG), 50% glucose was intravenously injected at a dose of 0.2 mL and intravenously infused at a rate of 2.2 mL/h. Rats were sacrificed after 24 h of glucose reperfusion. (B) Alda-1 treatment protocol. After 2 min of an isoelectric EEG state, Alda-1 was administered intravenously at a dose of 8.5 mg/kg with 50% glucose (Alda-1 group) or the same dose of dimethyl sulfoxide (DMSO) was administered (control group).