2-DE analysis of proteins induced by the <i>A</i>. <i>alternata</i> pathogen in resistant (R) and susceptible (S) leaves.

<p>Total protein (800 μg) was separated on 2D gels (pH 4–7) and stained with CBB R-350. Approximate molecular masses and pIs are indicated in the margins. Circles indicate the 43 proteins identified by MALDI-TOF-TOF/MS that changed in abundance more than 1.5-fold between controls and treated samples. The affected proteins are numbered, and the numbers correspond to the numbers in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0122233#pone.0122233.g003" target="_blank">Fig 3</a>. This figure represents three biological replicates.