(A) Survival rate, (B) parasitemia and (C to F) histological evaluation of cardiac tissue.

<p>A-B: Mice were infected with 100 blood trypomastigotes of the Colombian strain of <i>T</i>. <i>cruzi</i> and the (A) survival rate and (B) parasite load were evaluated 15, 30 or 45 days after infection. Each point represents the group mean and the vertical bars represent the standard error (n = 12 animals per group). C-F: Representative images of histological characteristics of infected animals after (C and D) haematoxylin and (E and F) eosin or anti-<i>T</i>. <i>cruzi</i> staining. The arrows indicate (C; inflammatory infiltrate; blue arrow; 45 dpi; 2,5x), myocytes vacuolization, myocarditis and fiber necrosis (D; 45 dpi; black arrow; 40x) and amastigote nests (E and F; green arrows; 45 dpi; 10x and 20x).