Comparison of DNA-marker methylation in benign prostatic hyperplasia (BPH) and high-risk PCa.

<p>The methylation of <i>RARB</i>, <i>GSTP1</i>, <i>APC</i>, <i>CCND2</i> and <i>PTGS2</i> was determined by QM-MSP in radical prostatectomy samples from 42 patients with BPH and from 147 (PCa1) and 71 (PCa2) patients with high-risk PCa. The data are shown in dot plots for the indicated genes (A-E, left graphs). Methylation levels of every gene in each PCa cohort were significantly higher than in the BPH group, as determined by the Mann-Whitney U-test (*, P<0.001). Receiver-operating-characteristic (ROC) analysis the methylation markers to discern BHP from high-risk prostate cancer samples (A-E; middle, PCa1 and right, PCa2), using the data displayed in the dot plots was performed. Grey line, median; AUC, area under the curve.