PTPN-2 depletion has no effect on Ang-1 mediated Tie-2 and Erk activation, and on expression of TJ proteins.

<p>Scramble and PTPN-2 siRNA transfected HBMECs were activated with 100ng/ml of Ang-1 for indicated times. (<b>A)</b> Immunoprecipitation of Tie-2 protein followed by immunoblotting with anti-PY-20 antibody; blot was reprobed with anti-Tie-2 antibody. (<b>B)</b> Whole cell lysates were immunoblotted with anti-Phospho-p44/42 (Erk1/2) (Thr202/Tyr204) and anti-Erk1/2 antibody. (<b>C)</b> Whole cell lysates were analyzed by Western blotting to study the expression of major TJ proteins with anti-ZO-1, anti-Occludin, anti-Claudin-5 and anti-PTPN-2 antibody. Actin used for loading control.