Effect of JAK inhibitors, PF956980 and PF1367550 on IFNγ and IFNγ + TNFα-stimulated phosphorylation of STAT-1 in human primary airway epithelial cells.

<p>Human primary airway epithelial cells were pre-treated for 1h with either 1μM PF956980 (PF956) or 1μM PF1367550 (PF136) followed by stimulation for 10 min with either IFNγ (10ng/ml) or IFNγ + TNFα. Cells were harvested and western blots performed for phosphorylated STAT-1 and total STAT-1 and quantified by densitometry. Data are representative of n = 4 independent experiments (panel a) and presented as mean ± SEM, n = 4 (panel b).