The effects of TAE226 on EGFR-related pathways in NSCLC cell lines.

<p>The NSCLC cell lines with EGFR-TKI-sensitive (exon 19 deletions) mutation (PC-9 and HCC827), cell lines with both EGFR-TKI-sensitive and-resistant (T790M) mutations (NCI-H1975 and RPC-9), and cell lines with wild-type EGFR (NCI-H1299 and NCI-H1819) were treated with TAE226 at several concentrations and western blotting analysis was performed. The phosphorylations of FAK and IGF-IR were not suppressed in all NSCLC cell lines tested. In contrast, the phosphorylations of EGFR and its downstream proteins, AKT and MAPK, were suppressed at lower concentration of TAE226 in <i>EGFR</i>-mutant cell lines regardless of the presence of T790M mutation compared with <i>EGFR</i>-wild-type cell lines. We incubated the membrane for Actin (42 KDa) after we incubated MAPK (42 and 44 KDa) and the stripping buffer was used for that membrane.