A clinical <i>P</i>. <i>aeruginosa</i> isolates that naturally secretes both ExoS and ExoT also causes formation of FOCI.

<p>Mice were infected with BL12+ S(R146A/E379A/E381A)bla and sacrificed at 12 and 18 hr post-infection. (Mice did not survive to 23 hr, preventing assessments at this later time point.) A) Proportion of total injected cells that were type I pneumocytes. Lung tissue sections encompassing an entire lung lobe were imaged to evaluate the localization of injected type I pneumocytes. Each data point represents a tissue section. One tissue section was analyzed per lung lobe, with at least two lobes analyzed per mouse and 3 mice per condition, for a total of at least 6 tissue sections per condition. Bars indicate medians. B) Representative image of a FOCI at 12 hr. Scale bar represents 50 μm.