Diagram of the recombinant baculovirus, AcHERV-GmCSF, and its expression in mammalian cells.

<p>(A) Diagram of Bacmid DNA of AcHERV-GmCSF containing HERV envelope and GmCSF genes under the transcriptional control of the AcMNPV PolH and hEF1α promoters, respectively. (B) Detection of mRNAs for GmCSF and 18s rRNA in baculovirus-infected 293TT cells by RT-PCR. Lane 1: Control for RT-PCR; lane 2: mock infection with AcMNPV in 293TT cells; lane 3: AcHERV-GmCSF-infected 293TT cells. (C) Quantification of GmCSF expression in baculovirus-infected 293TT cell lysates and supernatants by ELISA. NTC, not treated control; Mock, AcMNPV baculovirus-infected cells; AcHERV-GmCSF, AcHERV-GmCSF-infected cells. (D) Fluorescence micrograph of baculovirus-infected 293TT cells. Seventy-two hours after infection, the cells were incubated with a monoclonal mouse antibody against GmCSF followed by incubation with a FITC-conjugated goat anti-mouse IgG antibody. Mock, AcMNPV-infected cells; AcHERV-GmCSF, AcHERV-GmCSF-infected cells; Merge, merged image.