Reduced renal vessel area fraction in ob/ob mice at 21 weeks of age.

<p>Renal vessel area fraction was measured in lean (n = 11) and leptin-deficient (ob/ob, n = 10) mice at 21 weeks of age. A-D: Representative samples of kidney sections immunostained with Lectin I. A: Lectin I in lean mice. B: Computerized image analysis in lean mice. C: Lectin I in ob/ob mice. D: Computerized image analysis in ob/ob mice. E: Renal vessel area fraction was calculated as the mean area fraction of the identified small vessels compared to the total kidney tissue area examined. Values are presented as individual datapoints and means. Statistical analysis was performed using Mann-Whitney U-test and a p-value of less than 0.05 was considered significant.