Effect of high-fructose (HFr) supplementation (500 μM) on markers of oxidative stress and HO-1 and SIRT1 gene expressions in hepatocytes treated with and without CoPP (5 μM) and SnMP (5 μM).

<p>(A) Isoprostanes level in condition media of hepatocytes (B) heme content measurement in hepatocytes (C) superoxide measurement in hepatocytes. (D) HO-1 mRNA levels (E) SIRT-1 protein levels. (F) Effect of Biliverdin and Tempol on SIRT1 expression in fructose (Fr)-treated hepatocytes. Hepatocytes were treated with biliverdin (10 μM) and with Tempol (100 μM) in presence of fructose (500 μM) for 24 hours. Results are mean±SE, n = 6/group. * <i>p</i><0.05 vs CTR; # <i>p</i><0.05 vs HFr, + <i>p</i><0.05 vs HFr+CoPP.