Gemin2 upregulation by itself is deleterious to viability as well as motor function and enhances the <i>Gem3</i>^<i>BART</i> phenotype.

<p>(<b>A</b>) Left panel: Compared to the control genotype (<i>Mef2-GAL4/+</i>), pan-muscular overexpression of full-length Gemin2 (<i>Mef2-GAL4>Gem2</i>^<i>FL</i>) impairs flight early on during adulthood. In combination with <i>Gem3</i>^<i>BART</i>, Gemin2 overexpression (<i>Mef2-GAL4>Gem3</i>^<i>BART</i><i>+ Gem2</i>^<i>FL</i>) is lethal. Middle panel: Overexpression of the N-terminus of Gemin2 (<i>Mef2-GAL4>Gem2</i>^<i>∆C</i>) is uneventful with regards to motor function though when coupled with Gem3^<i>BART</i> (<i>Mef2-GAL4>Gem3</i>^<i>BART</i><i>+ Gem2</i>^<i>∆C</i>), it induces a progressive age-dependent decline in flight performance starting at day 15 post-eclosion. Right panel: Overexpression of the C-terminus of Gemin2 alone (<i>Mef-GAL4>Gem2</i>^<i>∆N</i>) has no effect on flight behaviour. In combination with <i>Gem3</i>^<i>BART</i> (<i>Mef-GAL4>Gem3</i>^<i>BART</i><i>+ Gem2</i>^<i>∆N</i>), it triggers lethality. Data presented are the mean ± S.E.M. of at least 4 independent experiments, and n ≥ 60 per genotype for each time point measured. Significance was tested by the unpaired t-test, and for all data, *p<0.05, ***p<0.001, and ****p<0.0001. (<b>B</b>) Expression of a low expressing in combination with a high expressing full-length <i>Gemin2</i> transgene in all tissues via the <i>1032</i>-GAL4 driver has a marginal impact on adult viability. The expression of two high expressing Gemin2 transgenes results in a dramatic reduction in adult viability at a culture temperature of 25°C, and leads to lethality at culture temperatures associated with maximal GAL4 activity (29°C). Data presented are the mean ± S.E.M. of at least 4 independent experiments, and n ≥ 100 per genotype at either culture temperature.