LILRB5 binds to HLA-B7 and HLA-B27 free heavy chains.

<p><b>A.</b> Isotype control (IgG2b), anti-FLAG (αFLAG) and eGFP staining of 293T (upper panels) and RBL cells (lower panels) transduced with LILRB5 lentivirus. <b>B</b> Left hand panel. Representative western blot of immunoprecipitates from cell lysates of 221B27 cells transduced with LILRB5 with isotype control MAb (IgG2a) or the class I heavy chain MAb HC10. Blots were probed with HRP conjugated anti-FLAG MAb. Right hand panel. Representative western blot of immunoprecipitates from cell lysates of RBLB27 and RBLB7 cells transduced with LILRB5 with isotype control MAb (IgG2a) or the class I heavy chain MAb HC10. Blots were probed with HRP conjugated anti-FLAG MAb. <b>C.</b> Upper panel. Representative western blot of immunoprecipitates with rabbit anti-FLAG antisera from cell lysates from parental RBL or RBL cells transduced with B27 and RBL cells transduced with LILRB5 and HLA-B27. Western blots were probed with the class I heavy chain MAb HC10 and HRP-conjugated-anti-mouse Igs. The asterisked band is non-specific and the position of monomeric class I heavy chain (M) is indicated. <b>D.</b> Upper panels. Representative western blots of anti-FLAG immunoprecipitates or cell lysates from RBLB27 and RBLB7 cells transduced with LILRA5 or LILRB5. Western blots of immunoprecipitates were probed with the class I heavy chain MAb HC10 and HRP-conjugated-anti-mouse Igs. Centre panels. Western blots of cell lysates were probed with HRP-conjugated mouse anti-FLAG antibody as indicated. Lower panel. Representative western blot of with HC10 MAb of cell lysates from RBL cells or RBL cells transduced with LILRB5 and HLA-B27 or HLA-B7. Representative blots from 1 of 2 independent experiments. All immunoprecipitates were resolved by SDS-PAGE performed under reducing conditions.