ATGL-1::GFP Expressed at a Higher Level in AMPK-deficient Animals during the Early Dauer Stage.

<p><b>(A)</b> Comparison of ATGL-1::GFP levels between control <i>daf-2</i> and <i>daf-2; aak(0)</i> animals during the early dauer stage. Dauer day 1 is defined as 72 hours after shifting to restrictive temperature (25°C) at the L1 stage (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130480#sec002" target="_blank">materials and methods</a>). Scale bar = 10μm. <b>(B)</b> Western blot analysis of GFP levels in control <i>daf-2</i> and <i>daf-2; aak(0)</i> mutant animals during early dauer stage. <b>(C)</b> Western blot analysis of endogenous ATGL-1 levels in control <i>daf-2</i> and <i>daf-2; aak(0)</i> mutant animals during early dauer stage. <b>(D)</b> Quantification of ATGL-1::GFP mRNA levels in control <i>daf-2</i> and <i>daf-2; aak(0)</i> mutant dauer day 4 animals using semi-quantitative RT-PCR. <i>act-1</i> was used as loading control.