Glucose utilization and reserve capacity in cultured CMs.

<p>Glucose utilization was assessed by examining percent change in ECAR and the reserve capacity assessed as percent change in OCR following FCCP challenge in the presence of indicated concentrations of GLP-1 or insulin at 70 nM. A typical seahorse plot representing changes in ECAR over time following acute treatment with 100 nM GLP-1 (maximal effective dose) or insulin optimal media (A) or suboptimal media (B). Percentage change in ECAR, 10 min post injection of GLP-1 (1, 10, 100 nM) or insulin with optimal media (C) and suboptimal media (D). Typical seahorse plots representing changes in OCR over time following acute treatment with 100 nM GLP-1 or insulin with optimal (E) or suboptimal (F) media. Percentage change in OCR, 80 min post injection of GLP-1 (1, 10, 100 nM) or insulin in optimal (G) and suboptimal (H) media. ECAR, extracellular acidification rate; OCR, oxygen consumption rate. Data are presented as mean±SEM of 3–5 replicates per treatment from 2–4 individual experiments. ***p<0.001, *p<0.05, vs Control.