Nucleolin is an IGFBP-5 binding partner.

<p>Following identification of nucleolin using mass spectrometry, interaction with IGFBP-5 was confirmed using co-precipitation. (A) Wild type IGFBP-5 binds nucleolin in fibroblasts from three different donors. (B) Reverse pull-down with nucleolin antibody confirms the interaction with IGFBP-5. GAPDH in input lysate was detected to confirm that lysate fractions with equivalent protein levels were used for the IP. (C) Co-localization of nucleolin and IGFBP-5 is detected using immunofluorescence (IGFBP-5: red; nucleolin: green).