The phylogeny and antigenic cartography of a panel of HAs from influenza vaccine strain components within the 2012–2014 TIVs.

<p><b>(a)</b> The phylogenic tree of HA genes of the influenza vaccine strains. <b>(b)</b> Heat map and dendrogram of the mPlex-Flu assay results using post-influenza infection ferret sera. Rows indicate the strain that the ferret was infected with to generate the strain-reactive serum, and columns indicate the recombinant influenza HA in the bead panel. Influenza strains are color coded based on the hierarchical clustering results, which were performed using a squared Euclidean distance measurement and cluster fusion level determined by the average cluster similarity metric. Note the segregation of the H3 strains by epoch (pre-2005 and post-2009). <b>(c)</b> Antigenic cartography generated with mPlex-Flu assay data. Relative antigenic distances are proportional to the distance between strains, reduced from 12- to 2-dimensions.