Epitope mapping of 297-D4, 144-A8, and α-BAP31 antibodies.

<p>(A) Schematic diagram of recombinant BAP31 fragments (residues 1–124, 1–158, 1–164, 1–207, and 1–246) used in this study. (B) Individual fusion proteins were expressed in bacteria as fusion proteins with GST tag at the N-terminus and stained with Coomassie brilliant blue after SDS-PAGE. (C-F) Western blot analysis of GST-BAP31 fusion proteins with anti-GST (C), 297-D4 (D), 144-A8 (E), and α-BAP31 (F) antibodies. The asterisks indicate partial degradation products of GST-BAP31 fusion proteins.