Additional file 2: of Mesenchymal stromal cells of osteosarcoma patients do not show evidence of neoplastic changes during long-term culture

Figure S1. Differentiation capacity and expression of membranous markers of MSCs. Representative examples are shown (40× magnification). A, chondrogenic differentiation was performed using cell pellet cultures and assessed using toluidine blue staining. Adipogenic differentiation was assessed using phase contrast microscopy (B) and Oil-red-O staining (C). Osteogenic differentiation was assessed using phase contrast microscopy (D) and Alizarin red staining (E). F, all samples expressed CD73, CD90 and CD105 (bold histograms) as determined by flow cytometry (isotype controle staining shown for comparison)