miRNA expression in prostate cell lines.

<p><b>A</b>) Heat-map of global miRNA profiles (≈750 miRNAs) in non-tumorigenic (RWPE-1, Normal), hyperplastic (BPH-1), androgen–dependent (LNCaP, AR^sens-PCa) or–independent (DU145 and PC3, AR^ins-PCa) prostate cancer cells. Red, over-expression; blue, under-expression, respectively. <b>B</b>) Scatterplots diagrams were performed between androgen–dependent and–independent PCa cells and between normal prostate cells and AR^sens PCa cells to select miRNAs with differential expression in either direction (fold-change) of at least 20 folds. A list of 41 miRNAs simultaneously altered in both comparisons was generated. <b>C)</b> Schematic diagram of selection criteria adopted to identify miRNAs potentially related to PCa. <b>D</b>) The eighteen miRNA signatures identified in <b>C</b> were verified by qPCR in the indicated prostate cells. All miRNAs except two (miR-577 and miR-886-3p) had mouse orthologs. Red, white and blue colors in the heat-map represent higher, equal or lower miRNA expression in samples respect to median miRNA value.