CRH promotes foam cell formation.

<p>(A) Murine peritoneal macrophages treated with or without CRH (10 nM) were incubated with or without oxLDL (50 μg/ml) for 18 hours in the presence of apoA1 (10 μg/ml). Oil-red-O (ORO) staining was performed. Representative pictures are provided (scale bar: 100 μm). (B) Measurement of ORO absorbance in the macrophage extracts. Fold-changes were plotted from triplicate experiments (C) Intracellular cholesterol measurement. After an 18-hour incubation with or without CRH (10 nM) and oxLDL (50 μg/ml), macrophages were lysed, and intracellular cholesterol was measured. All results are presented as mean ± SEM. The results were derived from triplicate experiments. <i>*** P < 0</i>.<i>001</i>, <i>n</i>.<i>s</i>.; <i>no statistical significance</i>.